Imagine a forest where a single tree can give rise to a million identical clones, or a rare orchid on the verge of extinction being brought back to life from a tiny piece of leaf. This is not science fiction; it is the reality of Plant Tissue Culture. Often referred to as "micropropagation," this technique is one of the most fascinating and impactful biotechnologies of the 20th century. It is the art and science of growing an entire plant from a minuscule piece of tissue—sometimes no larger than a speck of dust—under sterile, controlled conditions.
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In the realm of modern biotechnology, few techniques have revolutionized agriculture, horticulture, and scientific research as profoundly as Plant Tissue Culture (PTC) . From cloning endangered orchid species to producing disease-free potato plantlets, PTC is the backbone of micropropagation.
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The success of tissue culture depends on the composition of the growth medium. The most common medium is Murashige and Skoog (MS) Medium.
Key Components:
Sterilization: The medium is sterilized in an Autoclave at 121°C and 15 psi pressure for 15–20 minutes.
