Sequencher 414 - Portable

Raw current → Median filter → Event segmentation → Basecall (CNN+CRF) →  
Polishing (transformer model) → Alignment (minimap2 on device) →  
Variant calling (medaka-lite) → Output (FASTQ/BAM)

Latency: 3 seconds from molecule entry to called base (95th percentile).
Basecalling accuracy (single molecule, no polishing): 94.5% (Q15); after on-device RNN polishing: 98.9% (Q19.5).

Portable sequencers like the PS-414 enable a wide range of use cases where rapid, in‑field sequencing is valuable: portable sequencher 414

Advances that would enhance PS‑414‑style devices include: Raw current → Median filter → Event segmentation