Tecan Safire 2 Manual Pdf May 2026

Unlike filter-based readers, the Safire 2 uses dual monochromators (excitation and emission). Over time, the grating mechanism may drift. The manual provides the proprietary sequence for running the Calibration Plate (PN 30001200) and interpreting the acceptance criteria. This data is not available in the software help files.

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Even experienced scientists make mistakes. Here are three frequent errors that a careful read of the PDF prevents: Unlike filter-based readers, the Safire 2 uses dual

Error 1: Using the wrong plate bottom The Safire 2’s optics are optimized for clear-bottom, black-walled plates for fluorescence. The manual explicitly warns that white-walled plates cause cross-talk in fluorescence mode (due to light piping). Use white plates only for luminescence. This data is not available in the software help files

Error 2: Ignoring the temperature equilibration The manual states that the incubator (up to 42°C) requires a 10-minute equilibration time after the setpoint is reached. Running a kinetic assay immediately leads to a thermal gradient across the plate—visible as a diagonal drift in your data.

Error 3: Misunderstanding the Z-focus For cell-based assays in 96-well plates, the optimal focal height is typically 7-8mm from the bottom. The manual provides a stepwise protocol to run a “Z-focus scan” to find the ideal plane, avoiding out-of-focus cell monolayers.